DO16/1 Not Responding

I am having trouble with a DO Card not responding even when I force the values. I have no errors, I have three other cards in a 4 slot base, no matter where I move the DO card, it does not respond. I tried changing the card and still the same thing. I have even tried a new program ???

Try to connect the process data od the DO card as explained here: https://www.plcnext.help/te/PLCnext_Engineer/Assigning_process_data.htm